Journal: BMC genomics

Article Title: Expression profiles of microRNAs from lactating and non-lactating bovine mammary glands and identification of miRNA related to lactation.

doi: 10.1186/1471-2164-13-731

Figure Lengend Snippet: Figure 9 Expression of HK2 and STAT5 mRNA in the bovine mammary gland. Expression levels of HK2 and STAT5 in lactating and non-lactating bovine mammary glands were determined using real-time PCR. Gene fold changes were calculated using the 2−ΔCt

Article Snippet: Antibodies for STAT5 and β-actin were manufactured by Boster (China), and the HK2 antibody was purchased from Santa Cruz (USA).

Techniques: Expressing, Real-time Polymerase Chain Reaction

Journal: BMC genomics

Article Title: Expression profiles of microRNAs from lactating and non-lactating bovine mammary glands and identification of miRNA related to lactation.

doi: 10.1186/1471-2164-13-731

Figure Lengend Snippet: Figure 8 Putative miRNAs targeting gene 3’UTRs. (A) Pairing of bta-miR-141 with the bovine STAT5 3’UTR sequence. (B) A target seed region of the miRNA binding site was found within the bovine HK2 3’UTR sequence.

Article Snippet: Antibodies for STAT5 and β-actin were manufactured by Boster (China), and the HK2 antibody was purchased from Santa Cruz (USA).

Techniques: Sequencing, Binding Assay

Journal: BMC genomics

Article Title: Expression profiles of microRNAs from lactating and non-lactating bovine mammary glands and identification of miRNA related to lactation.

doi: 10.1186/1471-2164-13-731

Figure Lengend Snippet: Figure 10 STAT5 protein is regulated by miR-141. STAT5 and β-actin protein expression was determined by western blotting. Mac-T cells were transfected with an miR-141 inhibitor, an miR-141 mimic, an inhibitor negative control (INC) and a negative control (NC). Cell lysates were collected after 24 hours and analyzed by western blotting. Beta-actin served as the internal control. The protein fragment intensity was quantified using Imagpro-Plus software, and IOD was used to represent the protein level. Error bars represent SD. n=3. a-c means different superscripts differ (P<0.05).

Article Snippet: Antibodies for STAT5 and β-actin were manufactured by Boster (China), and the HK2 antibody was purchased from Santa Cruz (USA).

Techniques: Expressing, Western Blot, Transfection, Negative Control, Control, Software

Journal: medRxiv

Article Title: Expression of mechano-growth factor (MGF) in refractory overactive bladder

doi: 10.1101/2023.12.08.23299594

Figure Lengend Snippet: An antibody specific to the MGF E-peptide was used to detect expression of MGF protein by immunohistochemistry staining (a) and western blot (b-d) analysis of biopsy sections. In (a) 5µm sections of formalin-fixed OAB bladder biopsies were stained with anti-MGF (i) or secondary antibody only control (ii) and counterstained with haematoxylin. In (b-d) 30µg biopsy lysates were analysed by western blot for detection of MGF (∼15 kDa) and β-actin (42 kDa) as a loading control. L=size ladder. A representative blot is shown in (b) and a scatter plot showing quantification of MGF band intensity is shown in (c). The bar indicates mean relative MGF expression in control and OAB biopsies (0.22 and 0.51, respectively). In (d), the graph shows mean relative MGF expression +/- SEM in control/OAB biopsies in the three main sub-groups identified in : DES hi (n=6/5), VIM hi (n=1/6), DES&VIM hi (n=2/6). Statistical analysis in (c) and (d) was performed using the Mann-Whitney U test.

Article Snippet: Sections were stained using an anti-MGF E-peptide antibody (Merck Life Sciences #07-2108, rabbit polyclonal, 1/50 dilution) and HRP-tagged secondary (Vector Laboratories, BA-1100, 1/200 dilution), or secondary only control staining.

Techniques: Expressing, Immunohistochemistry, Staining, Western Blot, MANN-WHITNEY